Enzymatic conversion and hybrid capture target enrichment combine to optimize methylation sequencing.
Identifying DNA methylation through next-generation sequencing (NGS) provides important insights into gene expression changes. To prepare for NGS, scientists often treat methylated DNA samples with sodium bisulfite, which causes damage and leads to biased sequencing coverage. A new enzymatic conversion method coupled with target enrichment by hybrid capture offers a sensitive alternative approach that prepares DNA for methylome analysis in a variety of settings.